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Last updated: 2017/3/26
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MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B

  • Catalog # : MAB1637
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of MAPK1/MAPK3.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T202/T204 of human MAPK1/MAPK3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000-1:100000)
    Immunofluorescence
    Immunoprecipitation
    Western Blot (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl, pH 8.0 (10 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637). Western blot of MAPK1/MAPK3 activation in serum free DMEM, and EGF-100 ng/mL , 5min with 10% FBS in DMEM (4). Wells were equally loaded with 50 ug of whole cell lysate proteins/well. Western blot was performed and kindly provided by Dr. Martina Takacova from Intitute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HEK293 cells (A, untreated) and hydrogen peroxide treated cells (B) with MAPK1/MAPK3 (phospho T202/204) monoclonal antibody, clone G15-B (Cat # MAB1637) at 1:100 dilution.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5594
  • Gene Name:
  • MAPK1
  • Gene Alias:
  • ERK,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk
  • Gene Description:
  • mitogen-activated protein kinase 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174492,extracellular signal-regulated kinase 2,extracellular signal-regulated kinase-2,mitogen-activated protein kinase 2,protein tyrosine kinase ERK2
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
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