MAPK1/MAPK3 recombinant monoclonal antibody, clone 29B10
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: THP-1 whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: MCF-7 whole cell lysate; Lane 4: 293 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: PC-3 whole cell lysate; Lane 7: Mouse brain tissue lysate.
Immunohistochemistry
Immunohistochemistry image of MAPK1|MAPK3 recombinant monoclonal antibody, clone 29B10 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system.
Flow Cytometry
Overlay Peak curve showing Hela cells stained with MAPK1|MAPK3 recombinant monoclonal antibody, clone 29B10 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse MAPK1/MAPK3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MAPK1/MAPK3.
Theoretical MW (kDa)
Calculated MW: 42
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: THP-1 whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: MCF-7 whole cell lysate; Lane 4: 293 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: PC-3 whole cell lysate; Lane 7: Mouse brain tissue lysate.Immunohistochemistry
Immunohistochemistry image of MAPK1|MAPK3 recombinant monoclonal antibody, clone 29B10 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with MAPK1|MAPK3 recombinant monoclonal antibody, clone 29B10 (red line) at 1:50. -
Gene Info — MAPK1
Entrez GeneID
5594Protein Accession#
P27361;P28482Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Gene Info — MAPK3
Entrez GeneID
5595Protein Accession#
P27361;P28482Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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