MAPK3 (phospho T202) /MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5
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More Files
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Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 2: A549 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with EGF 100ng/ml/20mins) and Lane 4: HepG2 whole cell lysate (not treated) with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304).
Immunohistochemistry
Immunohistochemical staining of human glioma cancer with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304) (diluated at 1:100)
Immunofluorescence
Immunofluorescent staining of Hela cells with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAPK3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T202 of human MAPK3 and T185 of human MAPK1.
Theoretical MW (kDa)
Calculated MW: 42 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 2: A549 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with EGF 100ng/ml/20mins) and Lane 4: HepG2 whole cell lysate (not treated) with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304).Immunohistochemistry
Immunohistochemical staining of human glioma cancer with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304) (diluated at 1:100)Immunofluorescence
Immunofluorescent staining of Hela cells with MAPK3 (phospho T202) +MAPK1 (phospho T185) recombinant monoclonal antibody, clone 2D5 (Cat # RAB04304) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK3
Entrez GeneID
5595Protein Accession#
P27361Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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