Histone H3 pan monoclonal antibody
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More Files
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ChIP
ChIP assays were performed using human HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) 25 ug whole cell extracts of HeLa cells, (2) 1 ug of recombinant histone H2A, (3) 1 ug of recombinant histone H2B, (4) 1 ug of recombinant histone H3, and (5) 1 ug of recombinant histone H4.
Western Blot
Western Blot analysis of (1) 30 ug whole cell extracts of HeLa cells, (2) 30 ug whole cell extracts of K562 cells, (3) 30 ug whole cell extracts of MCF7 cells, (4) 30 ug whole cell extracts of U2OS cells, (5) 30 ug whole cell extracts of HepG2 cells, (6) 30 ug whole cell extracts of Jurkat cells, (7) 30 ug whole cell extracts of NIH3T3 cells, (8) 30 ug whole cell extracts of E14Tg2a mouse ES cells.
Immunofluorescence
Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (middle). The left panel shows staining of the nuclei with DAPI. A merge of the two stainings (right).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Histone H3 pan.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to unmodified sequence at C-terminus of Histone H3 pan.
Host
Mouse
Reactivity
Human, Mouse, Maize, Solanum lycopersicum, Populus
Form
Liquid
Purification
Protein A purification
Isotype
IgG3
Recommend Usage
Western Blot (1:1000)
ChIP (1-2 ug/CHIP)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using human HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) 25 ug whole cell extracts of HeLa cells, (2) 1 ug of recombinant histone H2A, (3) 1 ug of recombinant histone H2B, (4) 1 ug of recombinant histone H3, and (5) 1 ug of recombinant histone H4.Western Blot
Western Blot analysis of (1) 30 ug whole cell extracts of HeLa cells, (2) 30 ug whole cell extracts of K562 cells, (3) 30 ug whole cell extracts of MCF7 cells, (4) 30 ug whole cell extracts of U2OS cells, (5) 30 ug whole cell extracts of HepG2 cells, (6) 30 ug whole cell extracts of Jurkat cells, (7) 30 ug whole cell extracts of NIH3T3 cells, (8) 30 ug whole cell extracts of E14Tg2a mouse ES cells.Immunofluorescence
Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (middle). The left panel shows staining of the nuclei with DAPI. A merge of the two stainings (right). -
Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
P68431Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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Interactome
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Pathway
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Publication Reference
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Expression of the Parkinson's Disease-Associated Gene Alpha-Synuclein is Regulated by the Neuronal Cell Fate Determinant TRIM32.
Pavlou MA et al.
Molecular Neurobiology 2017 Aug; 54(6):4257.
Application：ChIP, Mouse, Mouse neural stem cells.
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Expression of the Parkinson's Disease-Associated Gene Alpha-Synuclein is Regulated by the Neuronal Cell Fate Determinant TRIM32.
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