Mouse monoclonal antibody raised against partial recombinant human MLH1.
Recombinant protein corresponding to amino acids 381-483 of human MLH1.
ELISA (1:10000) Immunofluorescence (1:200-1:1000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In ascitic fluid (0.03% sodium azide).
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum cancer (left) and ovarian cancer (right), showing nuclear localization with DAB staining.
Immunofluorescence staining of HeLa cells using MLH1 monoclonal antibody, clone 4C9C7 (Green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. showing cytoplasmic localization.
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
DNA mismatch repair protein Mlh1,MutL protein homolog 1