MLH1 monoclonal antibody, clone 4C9C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Line 1: HeLa, Line 2: MCF-7, Line 3: A549, Line 4: Jurkat, Line5: 2R75 and Line 6: COS cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum cancer (left) and ovarian cancer (right), showing nuclear localization with DAB staining.
Immunofluorescence
Immunofluorescence staining of HeLa cells using MLH1 monoclonal antibody, clone 4C9C7 (Green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. showing cytoplasmic localization.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human MLH1.
Immunogen
Recombinant protein corresponding to amino acids 381-483 of human MLH1.
Host
Mouse
Reactivity
Human, Monkey
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:10000)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic fluid (0.03% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Line 1: HeLa, Line 2: MCF-7, Line 3: A549, Line 4: Jurkat, Line5: 2R75 and Line 6: COS cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum cancer (left) and ovarian cancer (right), showing nuclear localization with DAB staining.Immunofluorescence
Immunofluorescence staining of HeLa cells using MLH1 monoclonal antibody, clone 4C9C7 (Green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. showing cytoplasmic localization.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MLH1
Entrez GeneID
4292Protein Accession#
P40692Gene Name
MLH1
Gene Alias
COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
Gene Description
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Gene Ontology
HyperlinkGene Summary
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
Other Designations
DNA mismatch repair protein Mlh1|MutL protein homolog 1
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Interactome
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Pathway
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Disease
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