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MLH1 monoclonal antibody, clone 4C9C7

  • Catalog # : MAB15272
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human MLH1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 381-483 of human MLH1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Monkey
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • ELISA (1:10000)
    Immunofluorescence (1:200-1:1000)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascitic fluid (0.03% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Line 1: HeLa, Line 2: MCF-7, Line 3: A549, Line 4: Jurkat, Line5: 2R75 and Line 6: COS cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum cancer (left) and ovarian cancer (right), showing nuclear localization with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HeLa cells using MLH1 monoclonal antibody, clone 4C9C7 (Green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. showing cytoplasmic localization.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4292
  • Gene Name:
  • MLH1
  • Gene Alias:
  • COCA2,FCC2,HNPCC,HNPCC2,MGC5172,hMLH1
  • Gene Description:
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
  • Gene Summary:
  • This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
  • Other Designations:
  • DNA mismatch repair protein Mlh1,MutL protein homolog 1
  • Related Disease
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