MLH1 recombinant monoclonal antibody, clone R02-2E8
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More Files
- More Functions
Western Blot
Western blot analysis of MLH1 in K562, C6, 3T3, Hela lysates using human MLH1 recombinant monoclonal antibody, clone R02-2E8 (Cat # RAB01708).
Immunocytochemistry
Immunocytochemistry analysis of MLH1(green) in Hela using human MLH1 recombinant monoclonal antibody, clone R02-2E8 (Cat # RAB01708), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human MLH1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MLH1
Theoretical MW (kDa)
Calculated MW: 85 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of MLH1 in K562, C6, 3T3, Hela lysates using human MLH1 recombinant monoclonal antibody, clone R02-2E8 (Cat # RAB01708).Immunocytochemistry
Immunocytochemistry analysis of MLH1(green) in Hela using human MLH1 recombinant monoclonal antibody, clone R02-2E8 (Cat # RAB01708), and DAPI(blue).Immunoprecipitation
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Gene Info — MLH1
Entrez GeneID
4292Protein Accession#
P40692Gene Name
MLH1
Gene Alias
COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
Gene Description
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Gene Ontology
HyperlinkGene Summary
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
Other Designations
DNA mismatch repair protein Mlh1|MutL protein homolog 1
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Interactome
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Pathway
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Disease
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