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Last updated: 2017/12/10

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alpha-Tubulin monoclonal antibody, clone RM113

  • Catalog # : MAB12827
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human alpha-Tubulin.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus region of human alpha-tubulin.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts to alpha-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
    Flow Cytometry
    Immunocytochemistry (1:200)
    Immunohistochemistry (1:200)
    Immunoprecipitation (1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of A431 cells using alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) at 1:1000 dilution.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry staining of A431 cells with alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7846
  • Gene Name:
  • TUBA1A
  • Gene Alias:
  • B-ALPHA-1,FLJ25113,LIS3,TUBA3
  • Gene Description:
  • tubulin, alpha 1a
  • Gene Summary:
  • Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq
  • Other Designations:
  • hum-a-tub1,hum-a-tub2,tubulin, alpha 3,tubulin, alpha, brain-specific
  • Gene Information
  • Gene Name:
  • TUBA1B
  • Gene Alias:
  • K-ALPHA-1
  • Gene Description:
  • tubulin, alpha 1b
  • Other Designations:
  • alpha tubulin,tubulin, alpha, ubiquitous
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