Products

Western Blot

Western blot analysis of Lane 1: HeLa, Lane 2: HEK293 and Lane 3: HepG2 lysates with TUBA1A recombinant monoclonal antibody, clone F2C (Cat # RAB03060).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Paraffin-embedded sections) of human cerebral cortex tissue with TUBA1A recombinant monoclonal antibody, clone F2C (Cat # RAB03060).

Immunofluorescence

Immunofluoresent staining of HeLa cells with TUBA1A recombinant monoclonal antibody, clone F2C (Cat # RAB03060).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of RAB03060 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing microtubule staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of with TUBA1A recombinant monoclonal antibody, clone F2C (Cat # RAB03060). (A) HepG2, (B) HeLa and (C) Hek293 cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03060 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Entrez GeneID

7846

Gene Name

TUBA1A

Gene Alias

B-ALPHA-1, FLJ25113, LIS3, TUBA3

Gene Description

tubulin, alpha 1a

Omim ID

602529 611603

Gene Ontology

Hyperlink

Gene Summary

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq

Other Designations

hum-a-tub1|hum-a-tub2|tubulin, alpha 3|tubulin, alpha, brain-specific