alpha-Tubulin monoclonal antibody, clone RM113
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A431 cells using alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) at 1:1000 dilution.
Immunocytochemistry
Immunocytochemistry staining of A431 cells with alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human alpha-Tubulin.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to C-terminus region of human alpha-tubulin.
Sequence
N/A
Specificity
This antibody reacts to alpha-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cells using alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) at 1:1000 dilution.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of A431 cells with alpha-Tubulin monoclonal antibody, clone RM113 (Cat # MAB12827) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
-
Gene Info — TUBA1A
Entrez GeneID
7846Gene Name
TUBA1A
Gene Alias
B-ALPHA-1, FLJ25113, LIS3, TUBA3
Gene Description
tubulin, alpha 1a
Gene Ontology
HyperlinkGene Summary
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq
Other Designations
hum-a-tub1|hum-a-tub2|tubulin, alpha 3|tubulin, alpha, brain-specific
-
Gene Info — TUBA1B
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Intralumenal docking of Connexin 36 channels in the ER isolates mis-trafficked protein.
Stephan Tetenborg, Viktoria Liss, Leonhard Breitsprecher, Ksenia Timonina, Anna Kotova, Alejandra Jesús Acevedo Harnecker, Chunxu Yuan, Eyad Shihabeddin, Fatemeh Ariakia, Guoting Qin, Cai Chengzhi, Karin Dedek, Georg Zoidl, Michael Hensel, John O'Brien.
The Journal of Biological Chemistry 2023 Sep; 105282.
Application：WB, Human, HEK293T cells.
-
Intralumenal docking of Connexin 36 channels in the ER isolates mis-trafficked protein.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com