Histone H3.3 monoclonal antibody, clone RM190
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More Files
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Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: recombinant Histone H3.3 and Lane 3: recombinant Histone H3.1 with Histone H3.3 monoclonal antibody, clone RM190 (Cat # MAB12805) at 1 ug/mL working concentration, showed a band of Histone H3.3 in HeLa cells and recombinant Histone H3.3; no cross activity with recombinant Histone H3.1.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H3.3 monoclonal antibody, clone RM190 (Cat# MAB12805) reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H3.3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Histone H3.3.
Sequence
N/A
Specificity
This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.2 ug/mL-1 ug/mL)
Western Blot (0.5 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: recombinant Histone H3.3 and Lane 3: recombinant Histone H3.1 with Histone H3.3 monoclonal antibody, clone RM190 (Cat # MAB12805) at 1 ug/mL working concentration, showed a band of Histone H3.3 in HeLa cells and recombinant Histone H3.3; no cross activity with recombinant Histone H3.1.Enzyme-linked Immunoabsorbent Assay
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Gene Info — H3F3A
Entrez GeneID
3020Protein Accession#
P84243Gene Name
H3F3A
Gene Alias
H3.3A, H3F3, MGC87782, MGC87783
Gene Description
H3 histone, family 3A
Omim ID
601128Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq
Other Designations
OTTHUMP00000035618|OTTHUMP00000035619|OTTHUMP00000035621
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Interactome
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