Western blot analysis of Lane 1: HeLa whole cell lysate, Lane 2: recombinant Histone H3.3 and Lane 3: recombinant Histone H3.1 with Histone H3.3 monoclonal antibody, clone RM190 (Cat # MAB12805) at 1 ug/mL working concentration, showed a band of Histone H3.3 in HeLa cells and recombinant Histone H3.3; no cross activity with recombinant Histone H3.1.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq