Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293 whole cell lysate and Lane 3: NIH/3T3 whole cell lysate with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172).
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172) (diluated at 1:100).
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H3.3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T3 of human histone H3.3.
Theoretical MW (kDa)
Calculated MW: 16 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293 whole cell lysate and Lane 3: NIH/3T3 whole cell lysate with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172).Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H3.3 (phospho T3) recombinant monoclonal antibody, clone 28H4 (Cat # RAB04172) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — H3F3A
Entrez GeneID
3020Protein Accession#
P84243Gene Name
H3F3A
Gene Alias
H3.3A, H3F3, MGC87782, MGC87783
Gene Description
H3 histone, family 3A
Omim ID
601128Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq
Other Designations
OTTHUMP00000035618|OTTHUMP00000035619|OTTHUMP00000035621
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Interactome
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