H3-3A recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:BT-20 whole cell lysate with H3-3A recombinant monoclonal antibody (Cat # RAB02773) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using H3-3A recombinant monoclonal antibody (Cat # RAB02773). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of BT-20 cells using H3-3A recombinant monoclonal antibody (Cat # RAB02773)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against H3-3A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant H3-3A.
Theoretical MW (kDa)
15
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Histone H3.3 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Chromatin immunoprecipitation (1:50-1:200)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
Western blot analysis of Lane1:BT-20 whole cell lysate with H3-3A recombinant monoclonal antibody (Cat # RAB02773) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using H3-3A recombinant monoclonal antibody (Cat # RAB02773). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of BT-20 cells using H3-3A recombinant monoclonal antibody (Cat # RAB02773)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — H3F3A
Entrez GeneID
3020Protein Accession#
P84243Gene Name
H3F3A
Gene Alias
H3.3A, H3F3, MGC87782, MGC87783
Gene Description
H3 histone, family 3A
Omim ID
601128Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq
Other Designations
OTTHUMP00000035618|OTTHUMP00000035619|OTTHUMP00000035621
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Interactome
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