Rabbit monoclonal antibody raised against synthetic acetyl peptide of human Histone H3 (acetyl K56).
A synthetic acetyl peptide corresponding to residues surrounding K56 of Histone H3.
This antibody reacts to Histone H3 acetylated at Lysine 56. No cross reactivity with other acetylated Lysines in histone H3.
Protein A purification
ELISA (0.5 ug/mL-1 ug/mL) Immunocytochemistry (0.5 ug/mL-2 ug/mL) Immunohistochemistry (1 ug/mL-10 ug/mL) Western Blot (1 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate and Lane 2: recombinant Histone H3.3 with Histone H3 (acetyl K56) monoclonal antibody, clone RM179 (Cat # MAB12782) at 1 ug/mL working concentration, showed a band of Histone H3 acetylated at Lysine 56.
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of HepG2 cells with Histone H3 (acetyl K56) monoclonal antibody, clone RM179 (Cat # MAB12782).
Immunocytochemical staining of HeLa cells treated with sodium butyrate with Histone H3 (acetyl K56) monoclonal antibody, clone RM179 (Cat # MAB12782) (Red). Actin filaments have been labeled with fluorescein phalloidin (Green).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq