Chromatin Immunoprecipitation (ChIP) analysis of HeLa cells with Histone H3 (dimethyl K79) monoclonal antibody, clone RM181 (Cat # MAB12781) at 5 ug/mL working concentration. Real-time PCR was performed using primers specific to the gene indicated.
Western blot analysis of Lane 1: recombinant Histone H3.3 and Lane 2: acid extracts of HeLa cell with Histone H3 (dimethyl K79) monoclonal antibody, clone RM181 (Cat # MAB12781) at 0.25 ug/mL working concentration, showed a band of Histone H3 dimethylated at Lysine 79.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq