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RPTOR monoclonal antibody, clone 1411CT316.2.151.34

  • Catalog # : MAB12359
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against human RPTOR.
  • Immunogen:
  • Recombinant protein corresponding to human RPTOR.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Immunohistochemistry (1:25)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela cell line lysates Lane 2: MCF-7 cell line lysates Lane 3: mouse C2C12 cell line lysates Lane 4: mouse NIH/3T3 cell line lysates Lane 5: rat C6 cell line lysates reacted with RPTOR monoclonal antibody (Cat # MAB12359) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human skeletal muscle section reacted with RPTOR monoclonal antibody (Cat # MAB12359) at 1:25 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • RPTOR
  • Gene Alias:
  • KOG1,Mip1
  • Gene Description:
  • regulatory associated protein of MTOR, complex 1
  • Gene Summary:
  • This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats,regulatory associated protein of mTOR
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