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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using RPTOR recombinant monoclonal antibody, clone 1B10 (Cat # RAB07495) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

57521

Protein Accession#

Q8N122

Gene Name

RPTOR

Gene Alias

KOG1, Mip1

Gene Description

regulatory associated protein of MTOR, complex 1

Omim ID

607130

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|regulatory associated protein of mTOR