Western blot analysis of cell lysates of HepG2 (40 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with PDCD4 monoclonal antibody , clone k4C1 (1 : 2000) (Cat # MAB1123). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
OTTHUMP00000020483,nuclear antigen H731,programmed cell death 4