PDCD4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PDCD4 polyclonal antibody (Cat # PAB25460) (1 ug/mL) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDCD4.
Immunogen
A synthetic peptide corresponding to internal region of human PDCD4.
Sequence
DSYKGTVDCVQAR
Host
Goat
Theoretical MW (kDa)
55
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PDCD4 polyclonal antibody (Cat # PAB25460) (1 ug/mL) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDCD4
Entrez GeneID
27250Protein Accession#
NP_055271.2;NP_663314.1;NP_001186421.1Gene Name
PDCD4
Gene Alias
H731, MGC33046, MGC33047
Gene Description
programmed cell death 4 (neoplastic transformation inhibitor)
Omim ID
608610Gene Ontology
HyperlinkGene Summary
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020483|nuclear antigen H731|programmed cell death 4
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Interactome
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Disease
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