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Last updated: 2017/2/19
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MAP2K2 monoclonal antibody, clone 7F5

  • Catalog # : MAB10792
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant MAP2K2.
  • Immunogen:
  • Recombinant protein corresponding to human MAP2K2.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) against PC-12 (1), Jurkat (2), HeLa (3) and NIH/3T3 (4) cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5605
  • Gene Name:
  • MAP2K2
  • Gene Alias:
  • FLJ26075,MAPKK2,MEK2,MKK2,PRKMK2
  • Gene Description:
  • mitogen-activated protein kinase kinase 2
  • Gene Summary:
  • The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • ERK activator kinase 2,MAP kinase kinase 2,MAPK/ERK kinase 2,dual specificity mitogen-activated protein kinase kinase 2,mitogen-activated protein kinase kinase 2, p45
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