MAP2K2 monoclonal antibody, clone 7F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) against PC-12 (1), Jurkat (2), HeLa (3) and NIH/3T3 (4) cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP2K2.
Immunogen
Recombinant protein corresponding to human MAP2K2.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) against PC-12 (1), Jurkat (2), HeLa (3) and NIH/3T3 (4) cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K2 monoclonal antibody, clone 7F5 (Cat # MAB10792) (green) and negative control (purple). -
Gene Info — MAP2K2
Entrez GeneID
5605Gene Name
MAP2K2
Gene Alias
FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
Gene Description
mitogen-activated protein kinase kinase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
Other Designations
ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|dual specificity mitogen-activated protein kinase kinase 2|mitogen-activated protein kinase kinase 2, p45
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Interactome
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Pathway
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Disease
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