FER monoclonal antibody, clone 5D2C4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) against NIH/3T3 (1) , A-549 (2) and SK-MEL-5 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissues using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) .
Immunofluorescence
Confocal immunofluorescence analysis of HeLa cells using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant FER.
Immunogen
Recombinant protein corresponding to human FER.
Host
Mouse
Theoretical MW (kDa)
95
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) against NIH/3T3 (1) , A-549 (2) and SK-MEL-5 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissues using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) .Immunofluorescence
Confocal immunofluorescence analysis of HeLa cells using FER monoclonal antibody, clone 5D2C4 (Cat # MAB10645) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FER
Entrez GeneID
2241Gene Name
FER
Gene Alias
TYK3
Gene Description
fer (fps/fes related) tyrosine kinase
Omim ID
176942Gene Ontology
HyperlinkGene Summary
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq
Other Designations
phosphoprotein NCP94
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Interactome
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Pathway
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Disease
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