FER (phospho Y402) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells cells, Lane 3: COS-7 cells with FER (phospho Y402) polyclonal antibody (Cat# PAB29298) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with FER (phospho Y402) polyclonal antibody (Cat# PAB29298) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human FER.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y402 of human FER.
Host
Rabbit
Theoretical MW (kDa)
85
Reactivity
Human
Specificity
FER (phospho Y402) polyclonal antibody detects endogenous levels of human FER only when phosphorylated at tyrosine 402.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells cells, Lane 3: COS-7 cells with FER (phospho Y402) polyclonal antibody (Cat# PAB29298) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with FER (phospho Y402) polyclonal antibody (Cat# PAB29298) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — FER
Entrez GeneID
2241Protein Accession#
P16591Gene Name
FER
Gene Alias
TYK3
Gene Description
fer (fps/fes related) tyrosine kinase
Omim ID
176942Gene Ontology
HyperlinkGene Summary
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq
Other Designations
phosphoprotein NCP94
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Interactome
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Pathway
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Disease
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