Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against HeLa (1), MCF-7 (2), Jurkat (3), T-47D (4), K-562 (5), A-431 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against recombinant protein.
Immunofluorescence analysis of 3T3-L1 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized. [provided by RefSeq