CCT2 monoclonal antibody, clone 5B5C4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against HeLa (1), MCF-7 (2), Jurkat (3), T-47D (4), K-562 (5), A-431 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against recombinant protein.
Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CCT2.
Immunogen
Recombinant protein corresponding to human CCT2.
Host
Mouse
Theoretical MW (kDa)
54
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against HeLa (1), MCF-7 (2), Jurkat (3), T-47D (4), K-562 (5), A-431 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) against recombinant protein.Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523).Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CCT2 monoclonal antibody, clone 5B5C4 (Cat # MAB10523) (blue) and negative control (red). -
Gene Info — CCT2
Entrez GeneID
10576Gene Name
CCT2
Gene Alias
99D8.1, CCT-beta, CCTB, MGC142074, MGC142076, PRO1633, TCP-1-beta
Gene Description
chaperonin containing TCP1, subunit 2 (beta)
Omim ID
605139Gene Ontology
HyperlinkGene Summary
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the gene described in this record have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
T-complex protein 1 subunit beta|T-complex protein 1, beta subunit|chaperonin containing TCP1, subunit 2|chaperonin containing t-complex polypeptide 1, beta subunit|chaperonin containing t-complex polypeptide 1, subunit 2
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Interactome
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Disease
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