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Last updated: 2017/6/25
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SMAD5 monoclonal antibody, clone 3H9

  • Catalog # : MAB10322
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SMAD5.
  • Immunogen:
  • Recombinant protein corresponding to human SMAD5.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 52
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) against HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K-562 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and lung cancer tissue (B) using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4090
  • Gene Name:
  • SMAD5
  • Gene Alias:
  • DKFZp781C1895,DKFZp781O1323,Dwfc,JV5-1,MADH5
  • Gene Description:
  • SMAD family member 5
  • Other Designations:
  • MAD homolog 5,MAD, mothers against decapentaplegic homolog 5,SMA- and MAD-related protein 5,SMAD, mothers against DPP homolog 5,mothers against decapentaplegic homolog 5,mothers against decapentaplegic, drosophila, homolog of, 5
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