Mouse monoclonal antibody raised against recombinant SMAD5.
Recombinant protein corresponding to human SMAD5.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and lung cancer tissue (B) using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) with DAB staining.
Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Jurkat cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green) and negative control (purple).
MAD homolog 5,MAD, mothers against decapentaplegic homolog 5,SMA- and MAD-related protein 5,SMAD, mothers against DPP homolog 5,mothers against decapentaplegic homolog 5,mothers against decapentaplegic, drosophila, homolog of, 5