Mouse monoclonal antibody raised against partial recombinant SYK.
Recombinant protein corresponding to amino acids 5-360 of human SYK.
Human, Mouse, Rat
This antibody reacts with protein tyrosine kinase p72Syk (Syk; Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (Fc epsilon RI).
The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blotting analysis (non - reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1) , RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3 , 4) . Lane 1 , 2 : Immunostaining with SYK monoclonal antibody, clone SYK - 01 (Cat # MAB0948) . Lane 3 , 4 : Immunostaining with anti - human Cytokeratin 18 (DC - 10) . Lane 5 : Negative control.
Immunofluorescence staining of SYK in human HeLa cell line using SYK monoclonal antibody, clone SYK-01 (Cat # MAB0948 1; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .
Immunofluorescence staining of SYK in human primary fibroblasts using SYK monoclonal antibody, clone SYK-01 (Cat # MAB0948 ; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue) .