Mouse monoclonal antibody raised against native ITGB2.
Immunogen:
Native purified ITGB2 from leukocytes of a patient suffering from a LGL-type leukemia.
Host:
Mouse
Theoretical MW (kDa):
90-95
Reactivity:
Human
Specificity:
This antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta 2 subunit; beta 2 integrin).
Form:
Liquid
Purification:
Differential precipitation and solid-phase chromatography methods
Isotype:
IgG1
Recommend Usage:
The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction:
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blotting analysis (non - reducing conditions) of whole cell lysate using ITGB2 monoclonal antibody, clone MEM - 48 (Cat # MAB0880) . Lane 1 : KG - 1a human acute myeloid leukemia cell line. Lane 2 : HPB - ALL human peripheral blood T cell leukemia cell line.
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Surface staining of human peripheral blood cells with ITGB2 monoclonal antibody, clone MEM - 48 (Cat # MAB0880) PE. Cells in the granulocyte gate were used for analysis.
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq