NFKBIA monoclonal antibody, clone 6A920(MAB0057)

NFKBIA monoclonal antibody, clone 6A920

Catalog # MAB0057

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Size:100 ug

Price: USD $ 531.00

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Datasheet
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Images

Application

Western Blot (Cell lysate)

Western blot analysis of NFKBIA. Using NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) at 2 ug/mL in (A) Daudi and (B) NIH/3T3 whole cell lysate.

Application

Western Blot (Cell lysate)

Jurkat cells were treated for 30 min with 100 ug/mL ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NFKBIA monoclonal antibody, clone 39A1413 (Cat # MAB0056) (lanes 1 & 2) or NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) (that recognizes both non-phospho and phosphorylated forms of IkBa) (lanes 3 & 4) and immunoreactivity was detected by ECL. The data shows that Cat # MAB0056 detects specifically the phosphorylated form of NFKBIA.

Specification

Product Description

Mouse monoclonal antibody raised against partial recombinant NFKBIA.

Immunogen

Recombinant protein corresponding to amino acids 32-291 of human NFKBIA.

Host

Mouse

Reactivity

Human, Mouse

Form

Liquid

Isotype

IgG1

Recommend Usage

The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS (0.05% BSA, 0.05% sodium azide)

Storage Instruction

Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications

Western Blot (Cell lysate)
Western blot analysis of NFKBIA. Using NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) at 2 ug/mL in (A) Daudi and (B) NIH/3T3 whole cell lysate.

Western Blot (Cell lysate)
Jurkat cells were treated for 30 min with 100 ug/mL ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NFKBIA monoclonal antibody, clone 39A1413 (Cat # MAB0056) (lanes 1 & 2) or NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) (that recognizes both non-phospho and phosphorylated forms of IkBa) (lanes 3 & 4) and immunoreactivity was detected by ECL. The data shows that Cat # MAB0056 detects specifically the phosphorylated form of NFKBIA.

Immunoprecipitation
Gene Info — NFKBIA

Entrez GeneID
4792

Gene Name

NFKBIA

Gene Alias

IKBA, MAD-3, NFKBI

Gene Description

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

Omim ID
164008

Gene Ontology
Hyperlink

Gene Summary

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM

Other Designations

IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
Interactome
- NFKBIA
Pathway
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Publication Reference
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  The Journal of Biological Chemistry 2020 Feb; 295(6):1575.
  
  Application：WB-Tr, Human, U-937 cells.
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  Application：WB-Ce, WB-Tr, Human, KB cells.
- p120-catenin mediates inflammatory responses in the skin.
  Mirna Perez-Moreno, Michael A Davis, Ellen Wong, H Amalia Pasolli, Albert B Reynolds, Elaine Fuchs.
  
  Cell 2006 Feb; 124(3):631.
  
  Application：WB-Ti, Mouse, Mouse epidermal lysates, Mouse skin.
- The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover.
  Lufen Chang, Hideaki Kamata, Giovanni Solinas, Jun-Li Luo, Shin Maeda, K Venuprasad, Yun-Cai Liu, Michael Karin.
  
  Cell 2006 Feb; 124(3):601.
  
  Application：WB-Ce, Mouse, Mouse hepatocytes.
- B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
  Min Yang, Hidenori Hase, Diana Legarda-Addison, Leena Varughese, Brian Seed, Adrian T Ting.
  
  Journal of Immunology 2005 Sep; 175(5):2814.
  
  Application：WB-Tr, Mouse, A20 B cells.
- Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1-polarizing program in dendritic cells.
  Giorgio Napolitani, Andrea Rinaldi, Francesco Bertoni, Federica Sallusto, Antonio Lanzavecchia.
  
  Nature Immunology 2005 Aug; 6(8):769.
  
  Application：WB-Ce, Human, Human monocyte-derived dendritic cells.
- The IkappaB kinase is a key factor in triggering influenza A virus-induced inflammatory cytokine production in airway epithelial cells.
  Daniela Bernasconi, Carla Amici, Simone La Frazia, Angela Ianaro, M Gabriella Santoro.
  
  The Journal of Biological Chemistry 2005 Jun; 280(25):24127.
  
  Application：WB-Tr, Human, A-549 cells.
- Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50.
  Yi-Feng Xia, Bu-Qing Ye, Yi-Dan Li, Jian-Guo Wang, Xiang-Jiu He, Xianfeng Lin, Xinsheng Yao, Dawei Ma, Arne Slungaard, Robert P Hebbel, Nigel S Key, Jian-Guo Geng.
  
  Journal of Immunology 2004 Sep; 173(6):4207.
  
  Application：WB-Ce, Human, HEK 293 cells.
- Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response.
  Simona Saccani, Ivan Marazzi, Amer A Beg, Gioacchino Natoli.
  
  The Journal of Experimental Medicine 2004 Jul; 200(1):107.
  
  Application：IP, WB-Tr, Human, Mouse, HEK 293T, NIH/3T3 cells.
- PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock.
  Takayuki Ikezoe, Yang Yang, David Heber, Hirokuni Taguchi, H Phillip Koeffler.
  
  Molecular Pharmacology 2003 Dec; 64(6):1521.
  
  Application：WB-Ce, Mouse, Raw 264.7 cells.
- NF-kappaB is required for apoptosis prevention during herpes simplex virus type 1 infection.
  Margot L Goodkin, Adrian T Ting, John A Blaho.
  
  Journal of Virology 2003 Jul; 77(13):7261.
  
  Application：WB-Tr, Human, HEp-2 cells.
- Modulation of NF-kappaB activity by exchange of dimers.
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  Molecular Cell 2003 Jun; 11(6):1563.
  
  Application：WB-Ce, WB-Tr, Human, HEK 293 cells, Human monocyte-derived dendritic cells.
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  Application：ChIP, EMSA, WB-Ce, Human, Dendritic cells.
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