VPS35 monoclonal antibody (M02J), clone 2D3
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More Files
- More Functions
Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in human colon.
Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in rat brain.
Western Blot (Cell lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in HeLa.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS35 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant VPS35.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
VPS35 (NP_060676, 697 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in human colon.Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in rat brain.Western Blot (Cell lysate)
VPS35 monoclonal antibody (M02J), clone 2D3. Western Blot analysis of VPS35 expression in HeLa.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS35 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — VPS35
Entrez GeneID
55737GeneBank Accession#
NM_018206Protein Accession#
NP_060676Gene Name
VPS35
Gene Alias
DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, MEM3
Gene Description
vacuolar protein sorting 35 homolog (S. cerevisiae)
Omim ID
606931Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
Other Designations
maternal-embryonic 3|vacuolar protein sorting 35
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