VPS35 recombinant monoclonal antibody, clone R03-6E1
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More Files
- More Functions
Western Blot
Western blot analysis of VPS35 in 3T3, mouse brain, rat brain, rat kidney, HepG2, U2OS, U251 lysates using VPS35 antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human VPS35.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human VPS35.
Theoretical MW (kDa)
Calculated MW: 92 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of VPS35 in 3T3, mouse brain, rat brain, rat kidney, HepG2, U2OS, U251 lysates using VPS35 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — VPS35
Entrez GeneID
55737Protein Accession#
Q96QK1Gene Name
VPS35
Gene Alias
DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, MEM3
Gene Description
vacuolar protein sorting 35 homolog (S. cerevisiae)
Omim ID
606931Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
Other Designations
maternal-embryonic 3|vacuolar protein sorting 35
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