VPS35 monoclonal antibody, clone AECH-22
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) Mouse kidney lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human VPS35.
Immunogen
A synthetic peptide corresponding to human VPS35.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat VPS35, in native form and recombinant. Superfamily members of VPS35 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) Mouse kidney lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — VPS35
Entrez GeneID
55737Protein Accession#
Q96QK1Gene Name
VPS35
Gene Alias
DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, MEM3
Gene Description
vacuolar protein sorting 35 homolog (S. cerevisiae)
Omim ID
606931Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
Other Designations
maternal-embryonic 3|vacuolar protein sorting 35
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