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Last updated: 2016/10/23
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PIAS3 monoclonal antibody (M03), clone 4F12

  • Catalog # : H00010401-M03
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant PIAS3.
  • Immunogen:
  • PIAS3 (NP_006090, 453 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00010401-M03
    Western Blot detection against Immunogen (36.52 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12.

    Lane 1: PIAS3 transfected lysate(67 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged PIAS3 is approximately 30ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi ( Cat # H00010401-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS3 monoclonal antibody (M03), clone 4F12 (Cat # H00010401-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Gene Name:
  • PIAS3
  • Gene Alias:
  • FLJ14651,ZMIZ5
  • Gene Description:
  • protein inhibitor of activated STAT, 3
  • Gene Summary:
  • This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000015586,zinc finger, MIZ-type containing 5
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