ATP6V0D1 monoclonal antibody (M01J), clone 2G12
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More Files
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Western Blot (Cell lysate)
ATP6V0D1 monoclonal antibody (M01J), clone 2G12. Western Blot analysis of ATP6V0D1 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of ATP6V0D1 expression in transfected 293T cell line by ATP6V0D1 monoclonal antibody (M01J), clone 2G12.
Lane 1: ATP6V0D1 transfected lysate (Predicted MW: 40.3 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ATP6V0D1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATP6V0D1 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (33.55 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ATP6V0D1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
ATP6V0D1 (NP_004682, 238 a.a. ~ 308 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFLN
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.55 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ATP6V0D1 monoclonal antibody (M01J), clone 2G12. Western Blot analysis of ATP6V0D1 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of ATP6V0D1 expression in transfected 293T cell line by ATP6V0D1 monoclonal antibody (M01J), clone 2G12.
Lane 1: ATP6V0D1 transfected lysate (Predicted MW: 40.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ATP6V0D1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATP6V0D1 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — ATP6V0D1
Entrez GeneID
9114GeneBank Accession#
NM_004691Protein Accession#
NP_004682Gene Name
ATP6V0D1
Gene Alias
ATP6D, ATP6DV, P39, VATX, VMA6, VPATPD
Gene Description
ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Omim ID
607028Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D|ATPase, H+ transporting, lysosomal 38kD, V0 subunit d|ATPase, H+ transporting, lysosomal, V0 subunit d1|H(+)-transporting two-sector ATPase, subunit D|V-ATPase 40 KDa accessory protein|V-
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Disease
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