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Last updated: 2016/10/23
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CDC45L monoclonal antibody (M01), clone 3F11-1F3

  • Catalog # : H00008318-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant CDC45L.
  • Immunogen:
  • CDC45L (AAH06232, 1 a.a. ~ 566 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2b Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00008318-M01
    Western Blot detection against Immunogen (88 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of CDC45L expression in transfected 293T cell line by CDC45L monoclonal antibody (M01), clone 3F11-1F3.

    Lane 1: CDC45L transfected lysate(65.6 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged CDC45L is approximately 3ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8318
  • Gene Name:
  • CDC45L
  • Gene Alias:
  • CDC45,CDC45L2,PORC-PI-1
  • Gene Description:
  • CDC45 cell division cycle 45-like (S. cerevisiae)
  • Gene Summary:
  • The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. [provided by RefSeq
  • Other Designations:
  • CDC45 (cell division cycle 45, S.cerevisiae, homolog)-like,CDC45-like,CDC45-related protein,cell division cycle 45-like 2,human CDC45
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