MAPK3 monoclonal antibody (M01), clone 3C9
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More Files
- More Functions
Western Blot (Cell lysate)
MAPK3 monoclonal antibody (M01), clone 3C9 Western Blot analysis of MAPK3 expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody (M01), clone 3C9.
Lane 1: MAPK3 transfected lysate(43.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of MAPK3 transfected lysate using anti-MAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPK3 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK3 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 25 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAPK3.
Immunogen
MAPK3 (AAH13992, 279 a.a. ~ 379 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAPK3 monoclonal antibody (M01), clone 3C9 Western Blot analysis of MAPK3 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody (M01), clone 3C9.
Lane 1: MAPK3 transfected lysate(43.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of MAPK3 transfected lysate using anti-MAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPK3 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK3 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 25 ug/ml] -
Gene Info — MAPK3
Entrez GeneID
5595GeneBank Accession#
BC013992Protein Accession#
AAH13992Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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