NFKBIA monoclonal antibody (M10), clone 3H4
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More Files
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Western Blot (Cell lysate)
NFKBIA monoclonal antibody (M10), clone 3H4. Western Blot analysis of NFKBIA expression in Hela S3 NE.
Western Blot (Transfected lysate)
Western Blot analysis of NFKBIA expression in transfected 293T cell line by NFKBIA monoclonal antibody (M10), clone 3H4.
Lane 1: NFKBIA transfected lysate (Predicted MW: 35.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFKBIA is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFKBIA on HeLa cell . [antibody concentration 20 ug/ml]
Western Blot detection against Immunogen (60.61 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant NFKBIA.
Immunogen
NFKBIA (AAH04983, 1 a.a. ~ 317 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (60.61 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NFKBIA monoclonal antibody (M10), clone 3H4. Western Blot analysis of NFKBIA expression in Hela S3 NE.Western Blot (Transfected lysate)
Western Blot analysis of NFKBIA expression in transfected 293T cell line by NFKBIA monoclonal antibody (M10), clone 3H4.
Lane 1: NFKBIA transfected lysate (Predicted MW: 35.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NFKBIA is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NFKBIA on HeLa cell . [antibody concentration 20 ug/ml] -
Gene Info — NFKBIA
Entrez GeneID
4792GeneBank Accession#
BC004983Protein Accession#
AAH04983Gene Name
NFKBIA
Gene Alias
IKBA, MAD-3, NFKBI
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Omim ID
164008Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
IkappaBalpha|nuclear factor of kappa light chain gene enhancer in B-cells
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Interactome
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Pathway
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Disease
- Adenocarcinoma
- Alcoholism
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
- Breast cancer
- Breast Neoplasms
- Bronchiolitis
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