MLH1 monoclonal antibody (M02), clone M1
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More Files
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Western Blot (Cell lysate)
MLH1 monoclonal antibody (M02), clone M1. Western Blot analysis of MLH1 expression in K-562.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MLH1 is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1. HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (108.9 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MLH1.
Immunogen
MLH1 (AAH06850, 1 a.a. ~ 756 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (108.9 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MLH1 monoclonal antibody (M02), clone M1. Western Blot analysis of MLH1 expression in K-562.Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MLH1 is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1. HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MLH1
Entrez GeneID
4292GeneBank Accession#
BC006850Protein Accession#
AAH06850Gene Name
MLH1
Gene Alias
COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
Gene Description
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Gene Ontology
HyperlinkGene Summary
This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. [provided by RefSeq
Other Designations
DNA mismatch repair protein Mlh1|MutL protein homolog 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Increased error-free DNA repair gene expression through reprogramming in human iPS cells.
Yoshimura Y.
Regenerative Therapy 2019 Jun; 11:101.
Application：WB-Ce, Human, Human dermal fibroblasts.
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Increased error-free DNA repair gene expression through reprogramming in human iPS cells.
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