LMNB1 monoclonal antibody (M01), clone 4B10
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More Files
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Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7(Cat # L024V1 ).
Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat ( Cat # L017V1 ).
Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3(Cat # L018V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.
Lane 1: LMNB1 transfected lysate(66.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (34.43 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant LMNB1.
Immunogen
LMNB1 (NP_005564.1, 107 a.a. ~ 186 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLAD
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.43 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7(Cat # L024V1 ).Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat ( Cat # L017V1 ).Western Blot (Cell lysate)
LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Transfected lysate)
Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.
Lane 1: LMNB1 transfected lysate(66.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LMNB1 is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — LMNB1
Entrez GeneID
4001GeneBank Accession#
NM_005573Protein Accession#
NP_005564.1Gene Name
LMNB1
Gene Alias
ADLD, LMN, LMN2, LMNB, MGC111419
Gene Description
lamin B1
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Fibrinopeptide A Induces Expression of C-Reactive Protein via the ROS-ERK1/2/ P38-NF-κB Signal Pathway in Vascular Smooth Muscle Cells.
Xu S, Zhao J, Liu J, Gou W.
Cellular Physiology and Biochemistry 2018 May; 47(1):266.
Application：WB-Ce, Rat, Vascular smooth muscle cells.
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Fibrinopeptide A Induces Expression of C-Reactive Protein via the ROS-ERK1/2/ P38-NF-κB Signal Pathway in Vascular Smooth Muscle Cells.
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