LIG4 monoclonal antibody (M02), clone 2D2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant LIG4.
Immunogen
LIG4 (AAH37491, 802 a.a. ~ 911 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (77)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LIG4 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — LIG4
Entrez GeneID
3981GeneBank Accession#
BC037491Protein Accession#
AAH37491Gene Name
LIG4
Gene Alias
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Gene Description
ligase IV, DNA, ATP-dependent
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
DNA joinase|DNA ligase IV|DNA repair enzyme|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase
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Interactome
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Pathway
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Disease
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