LIG4 recombinant monoclonal antibody, clone R08-3B4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: U251MG, Lane 3: HepG2 and Lane 4: MCF-7 lysates with LIG4 recombinant monoclonal antibody, clone R08-3B4 (Cat # RAB02320).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LIG4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human LIG4.
Theoretical MW (kDa)
Calculated MW: 104 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: U251MG, Lane 3: HepG2 and Lane 4: MCF-7 lysates with LIG4 recombinant monoclonal antibody, clone R08-3B4 (Cat # RAB02320). -
Gene Info — LIG4
Entrez GeneID
3981Protein Accession#
P49917Gene Name
LIG4
Gene Alias
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Gene Description
ligase IV, DNA, ATP-dependent
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
DNA joinase|DNA ligase IV|DNA repair enzyme|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase
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Interactome
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Pathway
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Disease
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