LIG4 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LIG4.
Immunogen
A synthetic peptide corresponding to internal of human LIG4.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total LIG4 protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using LIG4 polyclonal antibody (Cat # PAB17316).
Peptide "+" means "with peptide blocking". -
Gene Info — LIG4
Entrez GeneID
3981Protein Accession#
P49917Gene Name
LIG4
Gene Alias
-
Gene Description
ligase IV, DNA, ATP-dependent
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
DNA joinase|DNA ligase IV|DNA repair enzyme|polydeoxyribonucleotide synthase [ATP] 4|polynucleotide ligase|sealase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
The DNA sequence and analysis of human chromosome 13.
Dunham A, Matthews LH, Burton J, Ashurst JL, Howe KL, Ashcroft KJ, Beare DM, Burford DC, Hunt SE, Griffiths-Jones S, Jones MC, Keenan SJ, Oliver K, Scott CE, Ainscough R, Almeida JP, Ambrose KD, Andrews DT, Ashwell RI, Babbage AK, Bagguley CL, Bailey J, Bannerjee R, Barlow KF, Bates K, Beasley H, Bird CP, Bray-Allen S, Brown AJ, Brown JY, Burrill W, Carder C, Carter NP, Chapman JC, Clamp ME, Clark SY, Clarke G, Clee CM, Clegg SC, Cobley V, Collins JE, Corby N, Coville GJ, Deloukas P, Dhami P, Du
Nature 2004 Apr; 428(6982):522.
-
DNA ligase IV from HeLa cell nuclei.
Robins P, Lindahl T.
The Journal of Biological Chemistry 1996 Sep; 271(39):24257.
Application:WB-Re, Recombinant protein.
-
Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
Wei YF, Robins P, Carter K, Caldecott K, Pappin DJ, Yu GL, Wang RP, Shell BK, Nash RA, Schar P, et al..
Molecular and Cellular Biology 1995 Jun; 15(6):3206.
-
The DNA sequence and analysis of human chromosome 13.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com