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Last updated: 2017/6/25
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MAPK3 & RPS6KA3 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0354
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0354
    Representative image of Proximity Ligation Assay of protein-protein interactions between MAPK3 and RPS6KA3. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal antibody 1:1200 and anti-RPS6KA3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. MAPK3 rabbit purified polyclonal antibody (20 ug)
    2. RPS6KA3 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
  • Gene Information
  • Entrez GeneID:
  • 6197
  • Gene Name:
  • RPS6KA3
  • Gene Alias:
  • CLS,HU-3,ISPK-1,MAPKAPK1B,MRX19,RSK,RSK2,S6K-alpha3,p90-RSK2,pp90RSK2
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023036,insulin-stimulated protein kinase 1,mental retardation, X-linked 19,ribosomal protein S6 kinase, 90kD, polypeptide 3
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