RPS6KA3 & PDPK1 Protein Protein Interaction Antibody Pair
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More Files
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In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. A-549 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the RPS6KA3 protein, and the other against the PDPK1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. RPS6KA3 rabbit purified polyclonal antibody (100 ug)
2. PDPK1 mouse purified polyclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. A-549 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — PDPK1
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Gene Info — RPS6KA3
Entrez GeneID
6197Gene Name
RPS6KA3
Gene Alias
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations
OTTHUMP00000023036|insulin-stimulated protein kinase 1|mental retardation, X-linked 19|ribosomal protein S6 kinase, 90kD, polypeptide 3
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Interactome
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Pathway
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Disease
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