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Last updated: 2014/4/21
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BIOtech
BULK-new

EGFR polyclonal antibody

  • Catalog # : PAB7872
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 961-972 of human EGFR.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000)
    Immunocytochemistry (1:200)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot of human A-431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lane 1) or treated with lambda phosphatase (lane 2) then were probed with EGFR polyclonal antibody (Cat # PAB7872).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunocytochemical labeling in A-431 cells untreated (1) or treated with Calyculin A (50 nM) for 15 min (2). Cells were labeled with EGFR polyclonal antibody (Cat # PAB7872). The specificity of labeling was demonstrated with its respective blocking peptides EGFR polyclonal antibody (Cat # PAB7872) (3).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1956
  • Gene Name:
  • EGFR
  • Gene Alias:
  • ERBB,ERBB1,HER1,PIG61,mENA
  • Gene Description:
  • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • Gene Summary:
  • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
  • Other Designations:
  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
  • Interactome
  • Related Disease
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