STK4/STK3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STK4/STK3.
Immunogen
A synthetic peptide corresponding to residues surrounding T183 of human STK4/STK3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to STK4/STK3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with UV (15 mins), using STK4 polyclonal antibody (Cat # PAB18249).
Peptide "+" means "peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — STK3
Entrez GeneID
6788Protein Accession#
Q13188Gene Name
STK3
Gene Alias
FLJ90748, KRS1, MST2
Gene Description
serine/threonine kinase 3 (STE20 homolog, yeast)
Omim ID
605030Gene Ontology
HyperlinkGene Summary
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM
Other Designations
serine/threonine kinase 3 (Ste20, yeast homolog)
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Gene Info — STK4
Entrez GeneID
6789Protein Accession#
Q13188Gene Name
STK4
Gene Alias
DKFZp686A2068, KRS2, MST1, YSK3
Gene Description
serine/threonine kinase 4
Omim ID
604965Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq
Other Designations
OTTHUMP00000043418|dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))|kinase responsive to stress 2|mammalian sterile 20-like 1|yeast Ste20-like
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Interactome
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Pathway
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Disease
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Publication Reference
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Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.
Gómez EB, Espinosa JM, Forsburg SL.
Molecular and Cellular Biology 2005 Oct; 25(20):8887.
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Mapping of MST1 kinase sites of phosphorylation. Activation and autophosphorylation.
Glantschnig H, Rodan GA, Reszka AA.
The Journal of Biological Chemistry 2002 Nov; 277(45):42987.
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Caspase-catalyzed cleavage and activation of Mst1 correlates with eosinophil but not neutrophil apoptosis.
De Souza PM, Kankaanranta H, Michael A, Barnes PJ, Giembycz MA, Lindsay MA.
Blood 2002 May; 99(9):3432.
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Schizosaccharomyces pombe mst2+ encodes a MYST family histone acetyltransferase that negatively regulates telomere silencing.
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