MAP1LC3A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot on human brain lysate with MAP1LC3A polyclonal antibody (Cat # PAB15521).
Immunohistochemistry
Immunohistochemical staining of mouse liver with MAP1LC3A polyclonal antibody (Cat # PAB15521).
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Specification
Product Description
Rabbit polyclonal antibody raised against MAP1LC3A.
Immunogen
N-terminus of human MAP1LC3A.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat, Zebra fish
Specificity
Immunogen has 100% homology to rat and 97% homology to bovine. This antibody is useful for Western blot, where a band is seen ~14 KDa.
Form
Liquid
Recommend Usage
Flow Cytometry (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
Western Blot (1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.1% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot on human brain lysate with MAP1LC3A polyclonal antibody (Cat # PAB15521).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunohistochemical staining of mouse liver with MAP1LC3A polyclonal antibody (Cat # PAB15521).Flow Cytometry
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Gene Info — MAP1LC3A
Entrez GeneID
84557Protein Accession#
Q9H492Gene Name
MAP1LC3A
Gene Alias
LC3, LC3A, MAP1ALC3, MAP1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 alpha
Omim ID
601242Gene Ontology
HyperlinkGene Summary
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP1 light chain 3-like protein 1|MAP1A/1B light chain 3 A|MAP1A/MAP1B LC3 A|OTTHUMP00000030696|OTTHUMP00000030697|OTTHUMP00000030698|microtubule-associated proteins 1A/1B light chain 3
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Interactome
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Publication Reference
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Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells.
Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, Tokuhisa T, Ohsumi Y, Yoshimori T.
The Journal of Cell Biology 2001 Feb; 152(4):657.
Application：EM, IF, WB-Tr, Mouse, GFP24, GKR1, ES cells.
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Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells.
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