Rabbit polyclonal antibody raised against recombinant CASP9.
Recombinant protein corresponding to human CASP9.
Dog, Gerbils, Human, Mouse, Rat
Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000) Immunoprecipitation (1:50-1:200) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
In serum (0.05% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Formalin-fixed, paraffin-embedded human brain sections stained for Active/Cleaved CASP9 expression using CASP9 polyclonal antibody (Cat #PAB14669) at 1 : 2000. A and B. The pattern of CASP9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.
Formalin-fixed, paraffin-embedded dog brain sections stained for Active/Cleaved CASP9 expression using CASP9 polyclonal antibody (Cat #PAB14669) at 1 : 2000. A and B. The pattern of CASP9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq