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Last updated: 2014/11/23
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CDC2 (phospho Y15) polyclonal antibody

  • Catalog # : PAB12614
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y15 of human CDC2.
  • Sequence:
  • GTYGV
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody recognizes CDC2 (pY15) with a phosphorylated site at Tyrosine 15. It does not cross-react with non-phosphospecific peptide.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The A-431 cell lysate resolved onto 12% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2).
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : CDC2 (pY15, Phospho-specific).
    B : CDC2 (Paired Y15) (Non-phospho-speicfic).
    C : Non-related phospho-specific peptide.
    Followed were blotted at a 1 : 2000 dilution by :
    1. CDC2 (phospho Y15) polyclonal antibody (Cat # PAB12614).
    2. CDC2 polyclonal antibody (Cat # PAB12668).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 983
  • Gene Name:
  • CDC2
  • Gene Alias:
  • CDC28A,CDK1,DKFZp686L20222,MGC111195
  • Gene Description:
  • cell division cycle 2, G1 to S and G2 to M
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase
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