APOB monoclonal antibody, clone 6G6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) against human ApoB (aa : 3900-4110) recombinant protein. (Expected MW is 515.6 kDa)
Immunofluorescence
Immunofluorescence analysis of HepG2 cells using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with APOB monoclonal antibody, clone 6G6 (Cat # MAB10489).
Flow Cytometry
Flow cytometric analysis of serun using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green) and negative control (purple).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant APOB.
Immunogen
Recombinant protein corresponding to human APOB.
Host
Mouse
Theoretical MW (kDa)
516
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Western blot analysis using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) against human ApoB (aa : 3900-4110) recombinant protein. (Expected MW is 515.6 kDa)Immunofluorescence
Immunofluorescence analysis of HepG2 cells using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with APOB monoclonal antibody, clone 6G6 (Cat # MAB10489).Flow Cytometry
Flow cytometric analysis of serun using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green) and negative control (purple). -
Gene Info — APOB
Entrez GeneID
338Gene Name
APOB
Gene Alias
FLDB
Gene Description
apolipoprotein B (including Ag(x) antigen)
Gene Ontology
HyperlinkGene Summary
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq
Other Designations
OTTHUMP00000115994|apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com