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Last updated: 2014/7/21
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APOB monoclonal antibody, clone 6G6

  • Catalog # : MAB10489
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant APOB.
  • Immunogen:
  • Recombinant protein corresponding to human APOB.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 516
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) against human ApoB (aa : 3900-4110) recombinant protein. (Expected MW is 515.6 kDa)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with APOB monoclonal antibody, clone 6G6 (Cat # MAB10489).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of serun using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green) and negative control (purple).
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 338
  • Gene Name:
  • APOB
  • Gene Alias:
  • FLDB
  • Gene Description:
  • apolipoprotein B (including Ag(x) antigen)
  • Gene Summary:
  • This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000115994,apoB-100,apoB-48,apolipoprotein B,apolipoprotein B48
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