HMGCL recombinant monoclonal antibody, clone 22H11
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: PC-3 whole cell lysate; Lane3: HEK293 whole cell lysate; Lane 4: A549 whole cell lysate; Lane 5: HepG2 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of HMGCL recombinant monoclonal antibody, clone 22H11 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system.
Flow Cytometry
Overlay Peak curve showing Hela cells stained with HMGCL recombinant monoclonal antibody, clone 22H11 (red line) at 1:100.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HMGCL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HMGCL.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: PC-3 whole cell lysate; Lane3: HEK293 whole cell lysate; Lane 4: A549 whole cell lysate; Lane 5: HepG2 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of HMGCL recombinant monoclonal antibody, clone 22H11 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing Hela cells stained with HMGCL recombinant monoclonal antibody, clone 22H11 (red line) at 1:100. -
Gene Info — HMGCL
Entrez GeneID
3155Protein Accession#
P35914Gene Name
HMGCL
Gene Alias
HL
Gene Description
3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Omim ID
246450Gene Ontology
HyperlinkOther Designations
3-hydroxy-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)|3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)|OTTHUMP00000044830|hydroxymethylglutaricaciduria
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Interactome
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Pathway
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Disease
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