HMGCL monoclonal antibody (M01), clone 4F4-D1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.
Lane 1: HMGCL transfected lysate(34.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (61.49 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HMGCL.
Immunogen
HMGCL (AAH10570, 1 a.a. ~ 325 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (61.49 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.
Lane 1: HMGCL transfected lysate(34.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — HMGCL
Entrez GeneID
3155GeneBank Accession#
BC010570Protein Accession#
AAH10570Gene Name
HMGCL
Gene Alias
HL
Gene Description
3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Omim ID
246450Gene Ontology
HyperlinkOther Designations
3-hydroxy-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)|3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)|OTTHUMP00000044830|hydroxymethylglutaricaciduria
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Interactome
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Pathway
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Disease
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Publication Reference
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Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol.
Arnedo M, Menao S, Puisac B, Teresa-Rodrigo ME, Gil-Rodríguez MC, López-Viñas E, Gómez-Puertas P, Casals N, Casale CH, Hegardt FG, Pié J.
Journal of Lipid Research 2012 Oct; 53(10):2046.
Application：WB, Human, HEK 293 cells.
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Differential HMG-CoA lyase expression in human tissues provides clues about 3-hydroxy-3-methylglutaric aciduria.
Puisac B, Arnedo M, Casale CH, Ribate MP, Castiella T, Ramos FJ, Ribes A, Perez-Cerda C, Casals N, Hegardt FG, Pie J.
Journal of Inherited Metabolic Disease 2010 Aug; 33(4):405.
Application：WB, Human, Human tissues.
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Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol.
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