ITGAL recombinant monoclonal antibody, clone 2B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of THP-1 whole cell lysate with ITGAL recombinant monoclonal antibody, clone 2B2 (Cat # RAB07558).
Immunofluorescence
Immunofluorescent staining of THP-1 Cells with ITGAL recombinant monoclonal antibody, clone 2B2 (Cat # RAB07558), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ITGAL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ITGAL.
Theoretical MW (kDa)
Calculated MW: 129,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of THP-1 whole cell lysate with ITGAL recombinant monoclonal antibody, clone 2B2 (Cat # RAB07558).Immunofluorescence
Immunofluorescent staining of THP-1 Cells with ITGAL recombinant monoclonal antibody, clone 2B2 (Cat # RAB07558), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ITGAL
Entrez GeneID
3683Protein Accession#
P20701Gene Name
ITGAL
Gene Alias
CD11A, LFA-1, LFA1A
Gene Description
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Omim ID
153370Gene Ontology
HyperlinkGene Summary
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
LFA-1 alpha|antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide|integrin alpha L|integrin gene promoter|lymphocyte function-associated antigen 1
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Interactome
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Pathway
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Disease
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